Isolated mouse oviduct is capable of supporting development of bovine embryos [ 60 ]. Thus, our low IVF rate may not be due to a low competence of in vitro matured oocytes. In order to analyze the expression of osteopontin and ANP A during the oestrus cycle, equine oviducts were collected from slaughtered cyclic Welsh pony mares from our experimental stud. SOD values ranged from 7. After in vitro maturation, all equine COCs were denuded, rinsed and co-incubated for 2 to 3 hours with cell monolayers in an incubator. Furthermore, controversial results may hamper the practical use of SP on cryopreserved semen i. In the first trial, 6 and 4 in vivo matured intact COCs were co-incubated with or without porcine vesicles respectively.
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